Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
Identifieur interne : 000035 ( Main/Exploration ); précédent : 000034; suivant : 000036Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.
Auteurs : Siddheshwar Kshirsagar [Inde] ; Pankajkumar Waghmare [République populaire de Chine] ; Ganesh Saratale [Corée du Sud] ; Rijuta Saratale [Corée du Sud] ; Mayur Kurade [Corée du Sud] ; Byong-Hun Jeon [Corée du Sud] ; Sanjay Govindwar [Corée du Sud]Source :
- Applied biochemistry and biotechnology [ 1559-0291 ] ; 2020.
Abstract
We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars. Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp. KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes. Water hyacinth produced (U ml-1) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml-1) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp. KNU. Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids. The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml-1 and exoglucanase; 12.20 U ml-1), and by T. viride (filter paperase; 1.57 U ml-1). Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml-1). The highest xylanase (1129 U ml-1) was observed in T. viride + P. chrysosporium co-culture. This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.
DOI: 10.1007/s12010-020-03297-8
PubMed: 32206967
Affiliations:
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uniqKey="Saratale R" first="Rijuta" last="Saratale">Rijuta Saratale</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326</wicri:regionArea><wicri:noRegion>10326</wicri:noRegion></affiliation></author><author><name sortKey="Kurade, Mayur" sort="Kurade, Mayur" uniqKey="Kurade M" first="Mayur" last="Kurade">Mayur Kurade</name><affiliation wicri:level="1"><nlm:affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Earth Resources and Environmental Engineering, Hanyang 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spgovindwar@hanyang.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763</wicri:regionArea><wicri:noRegion>04763</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32206967</idno><idno type="pmid">32206967</idno><idno type="doi">10.1007/s12010-020-03297-8</idno><idno type="wicri:Area/Main/Corpus">000022</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000022</idno><idno type="wicri:Area/Main/Curation">000022</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000022</idno><idno type="wicri:Area/Main/Exploration">000022</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.</title><author><name sortKey="Kshirsagar, Siddheshwar" sort="Kshirsagar, Siddheshwar" uniqKey="Kshirsagar S" first="Siddheshwar" last="Kshirsagar">Siddheshwar Kshirsagar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, MS, 416004, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, MS, 416004</wicri:regionArea><wicri:noRegion>416004</wicri:noRegion></affiliation></author><author><name sortKey="Waghmare, Pankajkumar" sort="Waghmare, Pankajkumar" uniqKey="Waghmare P" first="Pankajkumar" last="Waghmare">Pankajkumar Waghmare</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong</wicri:regionArea><wicri:noRegion>Shandong</wicri:noRegion></affiliation></author><author><name sortKey="Saratale, Ganesh" sort="Saratale, Ganesh" uniqKey="Saratale G" first="Ganesh" last="Saratale">Ganesh Saratale</name><affiliation wicri:level="1"><nlm:affiliation>Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326</wicri:regionArea><wicri:noRegion>10326</wicri:noRegion></affiliation></author><author><name sortKey="Saratale, Rijuta" sort="Saratale, Rijuta" uniqKey="Saratale R" first="Rijuta" last="Saratale">Rijuta Saratale</name><affiliation wicri:level="1"><nlm:affiliation>Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326</wicri:regionArea><wicri:noRegion>10326</wicri:noRegion></affiliation></author><author><name sortKey="Kurade, Mayur" sort="Kurade, Mayur" uniqKey="Kurade M" first="Mayur" last="Kurade">Mayur Kurade</name><affiliation wicri:level="1"><nlm:affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763</wicri:regionArea><wicri:noRegion>04763</wicri:noRegion></affiliation></author><author><name sortKey="Jeon, Byong Hun" sort="Jeon, Byong Hun" uniqKey="Jeon B" first="Byong-Hun" last="Jeon">Byong-Hun Jeon</name><affiliation wicri:level="1"><nlm:affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea. bhjeon@hanyang.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763</wicri:regionArea><wicri:noRegion>04763</wicri:noRegion></affiliation></author><author><name sortKey="Govindwar, Sanjay" sort="Govindwar, Sanjay" uniqKey="Govindwar S" first="Sanjay" last="Govindwar">Sanjay Govindwar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea. spgovindwar@hanyang.ac.kr.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763</wicri:regionArea><wicri:noRegion>04763</wicri:noRegion></affiliation></author></analytic><series><title level="j">Applied biochemistry and biotechnology</title><idno type="eISSN">1559-0291</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars. Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp. KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes. Water hyacinth produced (U ml<sup>-1</sup>) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml<sup>-1</sup>) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp. KNU. Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids. The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml<sup>-1</sup> and exoglucanase; 12.20 U ml<sup>-1</sup>), and by T. viride (filter paperase; 1.57 U ml<sup>-1</sup>). Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml<sup>-1</sup>). The highest xylanase (1129 U ml<sup>-1</sup>) was observed in T. viride + P. chrysosporium co-culture. This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32206967</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0291</ISSN><JournalIssue CitedMedium="Internet"><Volume>191</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Applied biochemistry and biotechnology</Title><ISOAbbreviation>Appl Biochem Biotechnol</ISOAbbreviation></Journal><ArticleTitle>Composition of Synthesized Cellulolytic Enzymes Varied with the Usage of Agricultural Substrates and Microorganisms.</ArticleTitle><Pagination><MedlinePgn>1695-1710</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12010-020-03297-8</ELocationID><Abstract><AbstractText>We evaluated various agricultural lignocellulosic biomass and variety of fungi to produce cellulolytic enzymes cocktail to yield high amount of reducing sugars. Solid-state fermentation was performed using water hyacinth, paddy straw, corn straw, soybean husk/tops, wheat straw, and sugarcane bagasse using fungi like Nocardiopsis sp. KNU, Trichoderma reesei, Trichoderma viride, Aspergillus flavus, and Phanerochaete chrysosporium alone and in combination to produce cellulolytic enzymes. Water hyacinth produced (U ml<sup>-1</sup>) endoglucanase (51.13) and filter paperase (0.55), and corn straw produced (U ml<sup>-1</sup>) β-glucosidase (4.65), xylanase (113.32), and glucoamylase (41.27) after 7-day incubation using Nocardiopsis sp. KNU. Production of cellulolytic enzymes was altered due to addition of various nitrogen sources, metal ions, vitamins, and amino acids. The maximum cellulolytic enzymes were produced by P. chrysosporium (endoglucanase; 166.32 U ml<sup>-1</sup> and exoglucanase; 12.20 U ml<sup>-1</sup>), and by T. viride (filter paperase; 1.57 U ml<sup>-1</sup>). Among all, co-culture of T. reesei, T. viride, A. flavus, and P. chrysosporium showed highest β-glucosidase (17.05 U ml<sup>-1</sup>). The highest xylanase (1129 U ml<sup>-1</sup>) was observed in T. viride + P. chrysosporium co-culture. This study revealed the dependency on substrate and microorganism to produce good quality enzyme cocktail to obtain maximum reducing sugars.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Kshirsagar</LastName><ForeName>Siddheshwar</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, MS, 416004, India.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Waghmare</LastName><ForeName>Pankajkumar</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saratale</LastName><ForeName>Ganesh</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Saratale</LastName><ForeName>Rijuta</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>Research Institute of Biotechnology and Medical Converged Science, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kurade</LastName><ForeName>Mayur</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jeon</LastName><ForeName>Byong-Hun</ForeName><Initials>BH</Initials><AffiliationInfo><Affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea. bhjeon@hanyang.ac.kr.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Govindwar</LastName><ForeName>Sanjay</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Earth Resources and Environmental Engineering, Hanyang University, Seoul, 04763, South Korea. spgovindwar@hanyang.ac.kr.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>NRF-2017R1A2B2004143</GrantID><Agency>National Research Foundation of Korea</Agency><Country></Country></Grant><Grant><GrantID>HY201800000003220</GrantID><Agency>Hanyang University</Agency><Country></Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>03</Month><Day>23</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Appl Biochem Biotechnol</MedlineTA><NlmUniqueID>8208561</NlmUniqueID><ISSNLinking>0273-2289</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Enzymatic saccharification</Keyword><Keyword MajorTopicYN="N">Enzyme cocktail</Keyword><Keyword MajorTopicYN="N">Fungal consortium</Keyword><Keyword MajorTopicYN="N">Nocardiopsis sp. KNU</Keyword><Keyword MajorTopicYN="N">Water hyacinth</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>06</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>12</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>3</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32206967</ArticleId><ArticleId IdType="doi">10.1007/s12010-020-03297-8</ArticleId><ArticleId IdType="pii">10.1007/s12010-020-03297-8</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Corée du Sud</li><li>Inde</li><li>République populaire de Chine</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Kshirsagar, Siddheshwar" sort="Kshirsagar, Siddheshwar" uniqKey="Kshirsagar S" first="Siddheshwar" last="Kshirsagar">Siddheshwar Kshirsagar</name></noRegion></country><country name="République populaire de Chine"><noRegion><name sortKey="Waghmare, Pankajkumar" sort="Waghmare, Pankajkumar" uniqKey="Waghmare P" first="Pankajkumar" last="Waghmare">Pankajkumar Waghmare</name></noRegion></country><country name="Corée du Sud"><noRegion><name sortKey="Saratale, Ganesh" sort="Saratale, Ganesh" uniqKey="Saratale G" first="Ganesh" last="Saratale">Ganesh Saratale</name></noRegion><name sortKey="Govindwar, Sanjay" sort="Govindwar, Sanjay" uniqKey="Govindwar S" first="Sanjay" last="Govindwar">Sanjay Govindwar</name><name sortKey="Jeon, Byong Hun" sort="Jeon, Byong Hun" uniqKey="Jeon B" first="Byong-Hun" last="Jeon">Byong-Hun Jeon</name><name sortKey="Kurade, Mayur" sort="Kurade, Mayur" uniqKey="Kurade M" first="Mayur" last="Kurade">Mayur Kurade</name><name sortKey="Saratale, Rijuta" sort="Saratale, Rijuta" uniqKey="Saratale R" first="Rijuta" last="Saratale">Rijuta Saratale</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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